A high-complexity, multiplexed solution-phase assay for profiling protease activity on microarrays.

@article{Kozlov2008AHM,
  title={A high-complexity, multiplexed solution-phase assay for profiling protease activity on microarrays.},
  author={Igor A. Kozlov and Peter C. Melnyk and Johannes Hachmann and Anu Srinivasan and Melissa D Shults and Chanfeng Zhao and Joseph Musmacker and Nicholas A Nelson and D. Barker and Michal Lebl},
  journal={Combinatorial chemistry \& high throughput screening},
  year={2008},
  volume={11 1},
  pages={
          24-35
        }
}
We have developed a miniaturized and multiplexed solution assay for the measurement of protease activity in complex samples. This technology can accelerate research in functional proteomics and enable biologists to carry out multiplexed protease inhibitor screens on a large scale. The assay readout is based on Illumina's universal Sentrix BeadArrays. The peptide sequences that serve as protease substrates are conjugated to oligonucleotide sequences complementary to the oligo tags on randomly… 
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References

Evaluation of Different Chemical Strategies for Conjugation of Oligonucleotides to Peptides
TLDR
Novel assays for high-throughput detection of one or many kinases or proteases, each covalently linked to an oligonucleotide tag, and validated selected method during the successful generation of thousands oligon nucleotide-peptide conjugates are developed.