A heterogeneous tag-attachment to the homodimeric type 1 photosynthetic reaction center core protein in the green sulfur bacterium Chlorobaculum tepidum.

@article{Azai2011AHT,
  title={A heterogeneous tag-attachment to the homodimeric type 1 photosynthetic reaction center core protein in the green sulfur bacterium Chlorobaculum tepidum.},
  author={Chihiro Azai and Kwang Kim and Toru Kondo and Jiro Harada and Shigeru Itoh and Hirozo Oh-oka},
  journal={Biochimica et biophysica acta},
  year={2011},
  volume={1807 7},
  pages={803-12}
}
The 6xHis-tag-pscA gene, which was genetically engineered to express N-terminally histidine (His)-tagged PscA, was inserted into a coding region of the recA gene in the green sulfur bacterium Chlorobaculum tepidum (C. tepidum). Although the inactivation of the recA gene strongly suppressed a homologous recombination in C. tepidum genomic DNA, the mutant grew well under normal photosynthetic conditions. The His-tagged reaction center (RC) complex could be obtained simply by Ni(2+)-affinity… CONTINUE READING

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