A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF

  title={A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF},
  author={Shigeki Higashiyama and Jacob Abraham and J. Miller and JC Fiddes and Michael Klagsbrun},
  pages={936 - 939}
Macrophage-like U-937 cells secrete a 22-kilodalton heparin-binding growth factor that is mitogenic for BALB-3T3 fibroblasts and smooth muscle cells, but not endothelial cells. The amino acid sequence predicted from complementary DNA clones indicates that the mitogen is a new member of the epidermal growth factor (EGF) family. This heparin-binding EGF-like growth factor (HB-EGF) binds to EGF receptors on A-431 epidermoid carcinoma cells and smooth muscle cells, but is a far more potent mitogen… 

Heparin-binding EGF-like growth factor.

Structure and function of heparin-binding EGF-like growth factor (HB-EGF).

This review describes the primary structure of HB-EGF, as well as its processing, and summarizes its expression patterns, receptor-mediated signaling, and role in several important biological systems.

Heparin-binding epidermal growth factor-like growth factor regulates fibroblast growth factor-2 expression in aortic smooth muscle cells.

It is reported that HB-EGF treatment of serum-starved at aortic SMCs can induce fibroblast growth factor (FGF)-2 (basic FGF) gene expression but not FGF-1 (acidic FGF).

[Heparin-binding EGF-like growth factor (HB-EGF)].

The interaction of HB-EGF, via its heparin-binding domain, with cell surface heparan sulfate proteoglycan is essential for its optimal binding to the EGF receptor on bovine aortic smooth muscle cells, and hence for the optimal ability to stimulate migration.

Heparin-binding EGF-like growth factor is a mitogen for rat alveolar type II cells.

It is demonstrated that heparin-binding EGF-like growth factor is a mitogen for rat alveolar type II cells but appears to show species-specific differences with regard to its production by macrophages.

Epiregulin. A novel epidermal growth factor with mitogenic activity for rat primary hepatocytes.

It is suggested that epiregulin plays important roles in regulating the growth of epithelial cells and fibroblasts by binding to receptors for EGF-related ligands.



Human tumor cells synthesize an endothelial cell growth factor that is structurally related to basic fibroblast growth factor.

A human hepatoma cell line synthesizes an endothelial cell mitogen that is found to be mostly cell associated, and sequence analysis of tryptic fragments demonstrates that HDGF contains sequences that are homologous to both amino- terminal and carboxyl-terminal sequences of basic FGF.

Endothelial cell-derived basic fibroblast growth factor: synthesis and deposition into subendothelial extracellular matrix.

It is suggested that endothelium can store growth factors capable of autocrine growth promotion in two ways: by sequestering growth factor within the cell and by incorporating it into the underlying extracellular matrix.

Isolation and characterization of a macrophage-derived heparin-binding growth factor.

Human mononuclear cells were plated in culture, and the conditioned media of these cells were analyzed by heparin-Sepharose affinity chromatography to find macrophage-derived HBGF, a cationic heat-resistant polypeptide with a molecular weight in the range of 14-25 kDa that stimulated mitogenesis in 3T3 cells and stimulated the proliferation of vascular smooth muscle cells.

Expression and secretion of type beta transforming growth factor by activated human macrophages.

It is shown that cosecretion of TGF-beta and its monocyte/macrophage-derived binding protein may modulate growth factor action and that monocytic expression of other growth factor genes, such as the B chain of platelet-derived growth factor, is not constitutive and requires activation.

A radioreceptor assay for platelet-derived growth factor.

Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor.

A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth