A helical twist-induced conformational switch activates cleavage in the hammerhead ribozyme.

@article{Dunham2003AHT,
  title={A helical twist-induced conformational switch activates cleavage in the hammerhead ribozyme.},
  author={Christine M Dunham and James B Murray and William G. Scott},
  journal={Journal of molecular biology},
  year={2003},
  volume={332 2},
  pages={327-36}
}
We have captured the structure of a pre-catalytic conformational intermediate of the hammerhead ribozyme using a phosphodiester tether formed between I and Stem II. This phosphodiester tether appears to mimic interactions in the wild-type hammerhead RNA that enable switching between nuclease and ligase activities, both of which are required in the replicative cycles of the satellite RNA viruses from which the hammerhead ribozyme is derived. The structure of this conformational intermediate… CONTINUE READING
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tertiary structure : interhelical cross - linking of the hammerhead ribozyme

  • T. K. Stage-Zimmermann, O. C. Uhlenbeck
  • 2001

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