A guideline to family-wide comparative state-of-the-art quantitative RT-PCR analysis exemplified with a Brassicaceae cross-species seed germination case study.

@article{Graeber2011AGT,
  title={A guideline to family-wide comparative state-of-the-art quantitative RT-PCR analysis exemplified with a Brassicaceae cross-species seed germination case study.},
  author={Kai Graeber and Ada Linkies and Andrew T. A. Wood and Gerhard Leubner-Metzger},
  journal={The Plant cell},
  year={2011},
  volume={23 6},
  pages={2045-63}
}
Comparative biology includes the comparison of transcriptome and quantitative real-time RT-PCR (qRT-PCR) data sets in a range of species to detect evolutionarily conserved and divergent processes. Transcript abundance analysis of target genes by qRT-PCR requires a highly accurate and robust workflow. This includes reference genes with high expression stability (i.e., low intersample transcript abundance variation) for correct target gene normalization. Cross-species qRT-PCR for proper… CONTINUE READING

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