A guide to genome engineering with programmable nucleases

@article{Kim2014AGT,
  title={A guide to genome engineering with programmable nucleases},
  author={Hyongbum Henry Kim and Jin-Soo Kim},
  journal={Nature Reviews Genetics},
  year={2014},
  volume={15},
  pages={321-334}
}
Programmable nucleases — including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived from the bacterial clustered regularly interspaced short palindromic repeat (CRISPR)–Cas (CRISPR-associated) system — enable targeted genetic modifications in cultured cells, as well as in whole animals and plants. The value of these enzymes in research, medicine and biotechnology arises from their ability to induce site… 

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