A guide to choosing fluorescent proteins

  title={A guide to choosing fluorescent proteins},
  author={Nathan Christopher Shaner and Paul A Steinbach and Roger Tsien},
  journal={Nature Methods},
The recent explosion in the diversity of available fluorescent proteins (FPs) promises a wide variety of new tools for biological imaging. With no unified standard for assessing these tools, however, a researcher is faced with difficult questions. Which FPs are best for general use? Which are the brightest? What additional factors determine which are best for a given experiment? Although in many cases, a trial-and-error approach may still be necessary in determining the answers to these… CONTINUE READING
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