A group II intron RNA is a catalytic component of a DNA endonuclease involved in intron mobility

@article{Zimmerly1995AGI,
  title={A group II intron RNA is a catalytic component of a DNA endonuclease involved in intron mobility},
  author={Steven Zimmerly and Huatao Guo and Robert Eskest and Jian Yang and Philip S. Perlman and Alan M. Lambowitz},
  journal={Cell},
  year={1995},
  volume={83},
  pages={529-538}
}
The mobility (homing) of the yeast mitochondrial DNA group II intron al2 occurs via target DNA-primed reverse transcription at a double-strand break in the recipient DNA. Here, we show that the site-specific DNA endonuclease that makes the double-strand break is a ribonucleoprotein complex containing the al2-encoded reverse transcriptase protein and excised al2 RNA. Remarkably, the al2 RNA catalyzes cleavage of the sense strand of the recipient DNA, while the al2 protein appears to cleave the… 
Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double‐stranded DNA
TLDR
The results show how enzymes can use RNA and protein subunits cooperatively to recognize specific sequences in double‐stranded DNA.
Efficient integration of an intron RNA into double-stranded DNA by reverse splicing
TLDR
It is shown that over half of aI1 undergoes complete reverse splicing in vitro, thus integrating linear intron RNA directly into the DNA, which has implications for both intron mobility and evolution, and potential genetic engineering applications.
Interaction of a group II intron ribonucleoprotein endonuclease with its DNA target site investigated by DNA footprinting and modification interference.
TLDR
The results suggest that the protein first recognizes a small number of specific bases in the distal 5'-exon region of the DNA target site via major-groove interactions that promote DNA unwinding, enabling the intron RNA to base-pair to DNA top-strand positions -12 to +3 for reverse splicing.
Rules for DNA target-site recognition by a lactococcal group II intron enable retargeting of the intron to specific DNA sequences.
TLDR
This work analyzes DNA target-site requirements for the Lactococcus lactis Ll.LtrB group II intron in vitro and in vivo and suggests a model similar to yeast mtDNA introns, in which the intron-encoded protein first recognizes a small number of nucleotide residues in double-stranded DNA and causes DNA unwinding, enabling the introns RNA to base-pair with the DNA for reverse splicing.
RNA-Induced Changes in the Activity of the Endonuclease Encoded by the R2 Retrotransposable Element
TLDR
Analysis of the role of RNA in the DNA cleavage reaction has revealed that the binding of RNA induces the R2 protein to form a multimeric complex, and the specificity of this RNP complex is sufficiently great that it can find the 28S gene target site and conduct the TPRT reaction with total genomic DNA.
A reverse transcriptase/maturase promotes splicing by binding at its own coding segment in a group II intron RNA.
TLDR
The results suggest how the maturase functions in RNA splicing and support the hypothesis that the reverse transcriptase coding region was derived from an independent genetic element that was inserted into a preexisting group II intron.
Group II intron reverse transcriptase in yeast mitochondria. Stabilization and regulation of reverse transcriptase activity by the intron RNA.
TLDR
Results show that binding to intron-containing RNAs stabilizes and regulates the activity of p62, the major template for target DNA-primed reverse transcription in vitro is the reverse-spliced intron RNA, as found previously for aI1.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 28 REFERENCES
New reactions catalyzed by a group II intron ribozyme with RNA and DNA substrates
TLDR
Three novel reactions of the self-splicing group II intron bI1 (the first intron of the COB gene of yeast mitochondria) demonstrating its catalytic versatility are described, suggesting that excised group II introns might integrate directly into DNA without prior reverse transcription.
Group II intron mobility occurs by target DNA-primed reverse transcription
TLDR
In vitro experiments show that homing of the yeast mtDNA group II intron aI2 occurs by reverse transcription at a double-strand break in the recipient DNA, raising the possibility that mobile group II introns were ancestors of nuclear non-long terminal repeat retrotransposons and telomerases.
Group II intron RNA-catalyzed recombination of RNA in vitro.
We report the first evidence for a novel reaction mediated by the self-splicing yeast mitochondrial group II intron bl1; the site-specific recombination of RNA molecules in vitro. Upon incubation of
Reverse transcriptase activity associated with maturase-encoding group II introns in yeast mitochondria
TLDR
The results suggest that introns al1 and al2 of the yeast mtDNA cox1 gene are retroelements, which encode reverse transcriptases that have adapted to function in RNA splicing.
Mobile group II introns of yeast mitochondrial DNA are novel site-specific retroelements
TLDR
The results demonstrate that the yeast group II intron aI2 is a retroelement that uses novel mobility mechanisms that is accompanied by efficient, but highly asymmetric, coconversion of nearby flanking exon sequences.
Integration of group II intron bl1 into a foreign RNA by reversal of the self-splicing reaction in vitro
TLDR
Group II intron bI1, the first intron of the COB gene in the mitochondria of S. cerevisiae, is able to self-splice in vitro with the basic pathway similar to nuclear pre-mRNA splicing, implying that transposition of group II introns on the RNA level by reversal of the splicing reaction is, in principle, conceivable.
A maturase-encoding group IIA intron of yeast mitochondria self-splices in vitro.
TLDR
This is the first report of a maturase-encoding intron of either group I or group II that self-splices in vitro, and it requires much higher levels of Mg2+ and added salts for ready detection of splicing activity.
Reverse transcription of R2Bm RNA is primed by a nick at the chromosomal target site: A mechanism for non-LTR retrotransposition
TLDR
The open reading frame of the R2 element from Bombyx mori, R2Bm, in E. coli is expressed and it is shown that it encodes both sequence-specific endonuclease and reverse transcriptase activities.
Transposable group II introns in fission and budding yeast. Site-specific genomic instabilities and formation of group II IVS plDNAs.
TLDR
The hypothesis that transient IVS insertion at non-allelic sites followed by recombination can be viewed as a general molecular mechanism, applicable equally well to site-specific genomic instabilities involving splice-site borders of group II introns and to the formation of extra-genomic IVS plasmid DNAs (plDNAs).
Group I and group II introns
TLDR
This review summarizes current information about the structures of group I and group II introns, their RNA‐catalyzed reactions, the facilitation of RNA‐Catalyzed splicing by protein factors, and the ability of the introns to function as mobile elements.
...
1
2
3
...