A graphene oxide-based platform for the assay of RNA synthesis by RNA polymerase using a fluorescent peptide nucleic acid probe.

@article{Park2013AGO,
  title={A graphene oxide-based platform for the assay of RNA synthesis by RNA polymerase using a fluorescent peptide nucleic acid probe.},
  author={Joon Soo Park and Ahruem Baek and Il-soo Park and Bong-Hyun Jun and Dong-Eun Kim},
  journal={Chemical communications},
  year={2013},
  volume={49 80},
  pages={
          9203-5
        }
}
We report a simple, direct fluorometric assay based on graphene oxide (GO) for RNA polymerase-mediated RNA synthesis. In principle, fluorescent peptide nucleic acid (PNA) probes were designed, and annealed with RNA products and the resultant RNA-PNA hybrids induced the recovery of fluorescence intensity of the PNA probes adsorbed onto the GO surface. 
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A biosensor for the detection of single base mismatches in microRNA.
Graphene oxide quenches fluorescence corresponding to only a mismatched target due to selective denaturing of the thermo-unstable duplex composed of probe peptide nucleic acid and single base…
A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities
TLDR
A simple, rapid, and quantitative assay via a terminally capped DNA probe, reaction-specific-labeling and the fluorescence-quenching capability of graphene-oxide provides not only characterization of kinetic information but also the immediate classification of an exonuclease without needing to design a set of DNA substrates.
Fluorometric Detection of MicroRNA Using Isothermal Gene Amplification and Graphene Oxide.
TLDR
GO-based fluorescence detection coupled with isothermal gene amplification would be a simple and convenient method for the quantitative detection of miRNA.
Graphene Oxide as a Bifunctional Material toward Superior RNA Protection and Extraction.
TLDR
The nanobiological effects of total RNA/GO are investigated and it is demonstrated that the total RNA can be harbored on the surface of GO, thus resulting in a shield effect, enabling the discovery of GO as the potential next-generation RNase nanoinhibitor.
A base-modified PNA-graphene oxide platform as a turn-on fluorescence sensor for the detection of human telomeric repeats.
TLDR
The development of a simple homogeneous fluorescence turn-on method, which uses a unique combination of an environment-sensitive fluorescent nucleobase analogue, the superior base pairing property of PNA, and DNA-binding and fluorescence quenching properties of graphene oxide, to detect human telomeric DNA repeats of varying lengths is described.
Fluorescent sensors using DNA-functionalized graphene oxide
TLDR
The fundamental surface interactions between DNA and GO and the related fluorescence-quenching mechanism are summarized and the various sensor design strategies are critically compared.
Mechanism of DNA adsorption and desorption on graphene oxide.
TLDR
This study investigated the interaction of single- and double-stranded DNAs with GO by using a fluorescently labeled DNA probe and found that dsDNA can also be adsorbed on GO, albeit with lower affinity.
Single-Molecule FRET Studies of the Hybridization Mechanism during Noncovalent Adsorption and Desorption of DNA on Graphene Oxide.
TLDR
This investigation improves the fundamental understanding of the DNA hybridization dynamics on the GO surface, opening new windows in the field of biophysics as well as in sensing and therapeutic applications.
BSA as additive: A simple strategy for practical applications of PNA in bioanalysis.
Biosensors based on graphene oxide and its biomedical application☆
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