A gradient of template dependence defines distinct biological roles for family X polymerases in nonhomologous end joining.

@article{McElhinny2005AGO,
  title={A gradient of template dependence defines distinct biological roles for family X polymerases in nonhomologous end joining.},
  author={Stephanie A. Nick McElhinny and Jody M. Havener and Miguel Garcia-Diaz and Raquel Ju{\'a}rez and Katarzyna Bębenek and Barbara L Kee and Luis Blanco and T. A. Kunkel and Dale A. Ramsden},
  journal={Molecular cell},
  year={2005},
  volume={19 3},
  pages={357-66}
}
Three Pol X family members have been linked to nonhomologous end joining (NHEJ) in mammals. Template-independent TdT promotes diversity during NHEJ-dependent repair of V(D)J recombination intermediates, but the roles of the template-dependent polymerases mu and lambda in NHEJ remain unclear. We show here that pol mu and pol lambda are similarly recruited by NHEJ factors to fill gaps when ends have partially complementary overhangs, suggesting equivalent roles promoting accuracy in NHEJ. However… CONTINUE READING