A gradient-free method for the purification of infective dengue virus for protein-level investigations.

Abstract

Dengue virus (DENV) is a mosquito-transmitted flavivirus that infects approximately 100 million people annually. Multi-day protocols for purification of DENV reduce the infective titer due to viral sensitivity to both temperature and pH. Herein we describe a 5-h protocol for the purification of all DENV serotypes, utilizing traditional gradient-free… (More)
DOI: 10.1016/j.jviromet.2016.05.017

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