A glutamic acid 3‐methyltransferase encoded by an accessory gene locus important for daptomycin biosynthesis in Streptomyces roseosporus

@article{Nguyen2006AGA,
  title={A glutamic acid 3‐methyltransferase encoded by an accessory gene locus important for daptomycin biosynthesis in Streptomyces roseosporus},
  author={Kien Truc Giang Nguyen and David A. Kau and Jian-Qiao Gu and P. Dilkes Brian and Stephen K. Wrigley and Richard H. Baltz and Vivian P. W. Miao},
  journal={Molecular Microbiology},
  year={2006},
  volume={61}
}
In many peptide antibiotics, modified amino acids are important for biological activity. The amino acid 3‐methyl‐glutamic acid (3mGlu) has been found only in three cyclic lipopeptide antibiotics: daptomycin and the A21978C family produced by Streptomyces roseosporus, calcium‐dependent antibiotic produced by Streptomyces coelicolor and A54145 produced by Streptomyces fradiae. We studied the non‐ribosomal peptide synthetase genes involved in A21978C biosynthesis and the downstream genes, dptG… 
Development of a Genetic System for Combinatorial Biosynthesis of Lipopeptides in Streptomyces fradiae and Heterologous Expression of the A54145 Biosynthesis Gene Cluster
TLDR
A suite of mutant strains and plasmids was created for combinatorial biosynthesis efforts focused on modifying the A54145 peptide backbone to generate a compound with daptomycin antibacterial activity and activity in Streptococcus pneumoniae pulmonary infections.
Genomics and the ancient origins of the daptomycin biosynthetic gene cluster
  • R. H. Baltz
  • Medicine, Biology
    The Journal of Antibiotics
  • 2010
TLDR
Daptomycin is a clinically useful lipopeptide antibiotic produced by Streptomyces roseosporus, and the cyclized tridecapeptide contains three non-proteinogenic -amino acids: ornithine, 3-methyl-glutamic acid (3mGlu) and kynurinine (Kyn).
DptR2, a DeoR-type auto-regulator, is required for daptomycin production in Streptomyces roseosporus.
TLDR
It is reported that dptR2, encoding a DeoR-type regulator located close to the daptomycin biosynthesis gene cluster in S. roseosporus SW0702, is required for dptomycin production, but not for the expression of daptoniccin gene cluster, suggesting that DptR1 was not a pathway-specific regulator.
Non-ribosomal peptide synthetase module fusions to produce derivatives of daptomycin in Streptomyces roseosporus.
TLDR
This work explored module exchanges at nucleotide sequences encoding interpeptide linkers in dptD, a gene encoding a di-modular NRPS subunit that incorporates 3-methylglutamic acid and kynurenine into daptomycin, and found mutations causing amino acid substitutions, deletions or insertions in the inter-module linker had no negative effects on lipopeptide yields.
Chapter 20. Biosynthesis and genetic engineering of lipopeptides in Streptomyces roseosporus.
TLDR
Several of the daptomycin genes have been expressed from ectopic chromosomal loci under the transcriptional control of the strong constitutive ermEp* promoter, and recombinant strains produced high levels of lipopeptides, thus establishing a trans-complementation system for combinatorial biosynthesis.
Combinatorial biosynthesis of novel antibiotics related to daptomycin
TLDR
This study established a robust combinatorial biosynthesis platform to produce novel peptide antibiotics in sufficient quantities for antimicrobial screening and drug development.
Identification of novel mureidomycin analogues via rational activation of a cryptic gene cluster in Streptomyces roseosporus NRRL 15998
TLDR
This study provides a new way to activate cryptic gene cluster for the acquisition of novel antibiotics and will accelerate the exploitation of prodigious natural products in Streptomyces.
In silico aided metabolic engineering of Streptomyces roseosporus for daptomycin yield improvement
TLDR
The engineered strain demonstrated that the metabolic network based on in silico prediction would be accurate, reasonable, and practical for target gene identification and strain improvement in Streptomyces roseosporus.
Improvement of Daptomycin Production in Streptomyces roseosporus through the Acquisition of Pleuromutilin Resistance
Daptomycin, a cyclic lipopeptide antibiotic produced by Streptomyces roseosporus, displays potent activity against a variety of gram-positive pathogens. There is a demand for generating
Function of MbtH homologs in nonribosomal peptide biosynthesis and applications in secondary metabolite discovery
  • R. H. Baltz
  • Medicine, Biology
    Journal of Industrial Microbiology & Biotechnology
  • 2011
TLDR
Expression of MbtH-like proteins is important for a number of applications, including optimal production of native and genetically engineered secondary metabolites produced by mechanisms that employ NRPS enzymes and may serve as beacons to identify gifted actinomycetes and possibly other bacteria that encode multiple functional NRPS pathways for discovery of novel secondary metabolites by genome mining.
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