A glucoamylase::GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger.

@article{Gordon2000AGG,
  title={A glucoamylase::GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger.},
  author={C L Gordon and David Brian Archer and David J. Jeenes and John H. Doonan and Brian Wells and Anthony P. J. Trinci and G. D. Robson},
  journal={Journal of microbiological methods},
  year={2000},
  volume={42 1},
  pages={
          39-48
        }
}
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100 Citations
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100 Citations

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A study of the protein secretory pathway of Aspergillus niger using a glucoamylase-GFP fusion protein.
TLDR
The effect of various treatments that block protein secretion was visualized in Aspergillus niger using a strain expressing a glucoamylase-GFP fusion protein to show retargeting of the fusion protein from the secretory pathway to the vacuoles.
Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans
TLDR
The results indicate that increased expression may be a way for the cell to slow down secretion in order to cope with the increased protein load, and demonstrates a robust way to study the secretion pathway in filamentous fungi.
Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans
TLDR
The results indicate that increased expression may be a way for the cell to slow down secretion in order to cope with the increased protein load, and demonstrates a robust way to study the secretion pathway in filamentous fungi.
Toolkit for visualization of the cellular structure and organelles in Aspergillus niger.
TLDR
A standardized set of tools, consisting of an N-terminal GFP-actin fusion and codon optimized eforRed chromoprotein, are introduced, which constitute the first set of standardized A. niger biomarkers, which can be used to study morphology, intercellular trafficking, and secretion phenomena.
Transcriptome Analysis of Recombinant Protein Secretion by Aspergillus nidulans and the Unfolded-Protein Response In Vivo
TLDR
The effects of recombinant protein secretion on gene expression levels in Aspergillus nidulans are reported by directly comparing a bovine chymosin-producing strain with its parental wild-type strain in continuous culture by using expressed sequence tag microarrays.
Hyphal differentiation in the fungal mycelium
TLDR
It is concluded that two types of hyphae exist at the periphery of colonies; namely, thosehyphae that express glaA or aguA at a high level and those that express these genes at a low level, and the development of a fluorescent reporter system, which facilitates research aiming at hyphal differentiation in the mushroom forming fungus S. commune.
Plasmids for expression of heterologous proteins in Rhizopus oryzae
TLDR
A set of plasmid-based expression vectors that can be used for the production of heterologous proteins in R. oryzae are described and should be useful for overexpression of heterologicous proteins and potentially, metabolic engineering of Rhizopus strains.
Homologous expression of the feruloyl esterase B gene from Aspergillus niger and characterization of the recombinant enzyme.
Overproduction and characterization of xylanase B from Aspergillus niger.
TLDR
The xynB gene, which encodes endo-beta-1,4-xylanase XynB, in Aspergillus niger BRFM281 was amplified by RT-PCR using mRNA isolated from a culture containing sugar beet pulp as an inducer to produce the recombinant enzymeXynB with a six-histidine peptide fused to the carboxy end of the protein.
Heterologous Gene Expression in Filamentous Fungi: A Holistic View
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References

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