A glucoamylase::GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger.

@article{Gordon2000AGG,
  title={A glucoamylase::GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger.},
  author={C L Gordon and David Brian Archer and David J. Jeenes and John H. Doonan and Brian Wells and Anthony P. J. Trinci and G. D. Robson},
  journal={Journal of microbiological methods},
  year={2000},
  volume={42 1},
  pages={
          39-48
        }
}
A study of the protein secretory pathway of Aspergillus niger using a glucoamylase-GFP fusion protein.
TLDR
The effect of various treatments that block protein secretion was visualized in Aspergillus niger using a strain expressing a glucoamylase-GFP fusion protein to show retargeting of the fusion protein from the secretory pathway to the vacuoles.
Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans
TLDR
The results indicate that increased expression may be a way for the cell to slow down secretion in order to cope with the increased protein load, and demonstrates a robust way to study the secretion pathway in filamentous fungi.
Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans
TLDR
The results indicate that increased expression may be a way for the cell to slow down secretion in order to cope with the increased protein load, and demonstrates a robust way to study the secretion pathway in filamentous fungi.
Toolkit for visualization of the cellular structure and organelles in Aspergillus niger.
TLDR
A standardized set of tools, consisting of an N-terminal GFP-actin fusion and codon optimized eforRed chromoprotein, are introduced, which constitute the first set of standardized A. niger biomarkers, which can be used to study morphology, intercellular trafficking, and secretion phenomena.
Transcriptome Analysis of Recombinant Protein Secretion by Aspergillus nidulans and the Unfolded-Protein Response In Vivo
TLDR
The effects of recombinant protein secretion on gene expression levels in Aspergillus nidulans are reported by directly comparing a bovine chymosin-producing strain with its parental wild-type strain in continuous culture by using expressed sequence tag microarrays.
Hyphal differentiation in the fungal mycelium
TLDR
It is concluded that two types of hyphae exist at the periphery of colonies; namely, thosehyphae that express glaA or aguA at a high level and those that express these genes at a low level, and the development of a fluorescent reporter system, which facilitates research aiming at hyphal differentiation in the mushroom forming fungus S. commune.
Plasmids for expression of heterologous proteins in Rhizopus oryzae
TLDR
A set of plasmid-based expression vectors that can be used for the production of heterologous proteins in R. oryzae are described and should be useful for overexpression of heterologicous proteins and potentially, metabolic engineering of Rhizopus strains.
Overproduction and characterization of xylanase B from Aspergillus niger.
TLDR
The xynB gene, which encodes endo-beta-1,4-xylanase XynB, in Aspergillus niger BRFM281 was amplified by RT-PCR using mRNA isolated from a culture containing sugar beet pulp as an inducer to produce the recombinant enzymeXynB with a six-histidine peptide fused to the carboxy end of the protein.
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References

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TLDR
The results indicate that the GLA::sGFP fusion constructs can be used as convenient fluorescent markers to study the dynamics of protein secretion in vivo and as a tool in the isolation of mutants in the general secretory pathway.
Study of the glucoamylase promoter in Aspergillus niger using green fluorescent protein.
TLDR
An Aspergillus niger strain expressing a red-shifted green fluorescent protein (GFP) in the cytoplasm under the control of the glucoamylase promoter (PgIaA) was characterized with respect to its physiology and morphology and it was shown that GFP can be used as a marker of cellular activity in this type of cultivation.
Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels
TLDR
Results indicated that transcription elongation or premature termination was not the reason for the generation of truncated mRNAs, and a glaA fusion with the synthetic aglA gene resulted in a 25-fold increase in the mRNA level and, as a consequence, a similar increase inThe alpha-galactosidase protein level.
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TLDR
This review will focus on the use of filamentous fungi for the production of heterologous proteins, especially non-fungal, proteins and the effect of gene-fusion strategies will be reviewed.
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TLDR
Results indicate that glucoamylase secretion is located at the tips of growing hyphae only, and that Aspergillus niger growth and secretion are confined to the periphery of colonies.
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TLDR
The results indicate that ech42 is expressed before contact of T. harzianum with R. solani and its induction is triggered by soluble chitooligosaccharides produced by constitutive activity of CHIT42 and/or other chitinolytic enzymes.
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