A global benchmark study using affinity-based biosensors.

  title={A global benchmark study using affinity-based biosensors.},
  author={Rebecca L. Rich and Giuseppe A Papalia and Peter J Flynn and Jamie Furneisen and John Quinn and Joshua S. Klein and Phini S Katsamba and M. B. Waddell and Michael Scott and Joshua Thompson and Judie Berlier and Schuyler Corry and Mireille Baltzinger and Gabrielle Zeder-Lutz and Andreas Schoenemann and Anca Clabbers and S{\'e}bastien Wieckowski and M Margarita Murphy and Phillip Page and Thomas E. Ryan and Jay L. Duffner and Tanmoy Ganguly and John A. Corbin and Satyen Gautam and Gregor Anderluh and Andrej Bavdek and Dana Reichmann and Satya P. Yadav and Eric L. Hommema and E. Saint Pol and Andrew J. Drake and Scott L Klakamp and Trevor L. Chapman and Dawn Kernaghan and Ken Miller and Jason T. Schuman and Kevin Lindquist and Kara Herlihy and Michael B. Murphy and Richard N. Bohnsack and Bruce A. Andrien and Pietro Brandani and Danny Terwey and Rohn L Millican and Ryan J Darling and Liann Wang and Quincy L Carter and Joe E. Dotzlaf and Jacinto L{\'o}pez-Sagaseta and Islay Campbell and Paola Torreri and Sylviane Hoos and Patrick England and Yang Liu and Yasmina Noubia Abdiche and Daniel S. Malashock and Alanna C. Pinkerton and Melanie Wong and Eileen M. Lafer and Cynthia S. Hinck and Kevin Thompson and Carmelo Di Primo and Alison Joyce and Jonathan Daniel Brooks and Federico Torta and Anne Birgitte Bagge Hagel and Janus Kristian Krarup and Jesper Pass and M{\^o}nica Freitas Ferreira and Sergei Shikov and Malgorzata G Mikolajczyk and Yuki Abe and Gaetano Barbato and Anthony M. Giannetti and Ganeshram Krishnamoorthy and Bianca Beusink and Daulet Satpaev and Tiffany Tsang and Eric Fang and James Partridge and Stephen G. Brohawn and James R. van Horn and Otto Pritsch and Gonzalo Obal and Sanjay Nilapwar and Ben Busby and Gerardo Guti{\'e}rrez-S{\'a}nchez and R. Das Gupta and Sylvie Can{\'e}pa and Krista Witte and Z. Nikolovska-Coleska and Yun Hee Cho and Roberta D'Agata and Kristian H Schlick and Rosy Calvert and Eva Ma Mart{\'i}nez Mu{\~n}oz and Mar{\'i}a Jos{\'e} Hern{\'a}iz and Tsafir Bravman and Monica Dines and M Yang and Agnes Pusk{\'a}s and Erica E. Boni and Jiejin Li and Martin A Wear and Asya V Grinberg and Jason Baardsnes and Olan Dolezal and Melicia R Gainey and Henrik Anderson and Jinlin Peng and M. Lewis and Peter Spies and Quyhn Trinh and Sergei Bibikov and Jill A Raymond and Mohammed Yousef and Vidya Chandrasekaran and Yuguo Feng and Anne W Emerick and Suparna Mundodo and Rejane L. Guimar{\~a}es and Katy McGirr and Y. Li and Heather D Hughes and Hubert Mantz and Rostislav {\vS}krabana and M. R. Witmer and Joshua Ballard and Loic Martin and Petr Skl{\'a}dal and George Korza and Ite A. Laird-Offringa and Charlene S. Lee and Abdelkrim Khadir and Frank Podlaski and Phillippe Neuner and Julie Rothacker and Ashique Rafique and Nico Dankbar and Peter Kainz and Erk T Gedig and Momchilo Vuyisich and Christina Boozer and Nguyen Thi Thanh Ly and Mark Toews and Aykut Uren and Oleksandr Kalyuzhniy and Kenneth D Lewis and Eugene G Chomey and Brian J. Pak and David G. Myszka},
  journal={Analytical biochemistry},
  volume={386 2},
To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several… CONTINUE READING