A genomic toolkit to investigate kinesin and myosin motor function in cells

@article{Maliga2013AGT,
  title={A genomic toolkit to investigate kinesin and myosin motor function in cells},
  author={Zoltan Maliga and Magno Junqueira and Yusuke Toyoda and Andreas W. Ettinger and Felipe Mora-Berm{\'u}dez and Robin W. Klemm and Andrej Vasilj and Elaine Guhr and Itziar Ibarlucea-Benitez and Ina Poser and Ezio Bonifacio and Wieland B. Huttner and Andrej Shevchenko and Anthony A. Hyman},
  journal={Nature Cell Biology},
  year={2013},
  volume={15},
  pages={325-334}
}
Coordination of multiple kinesin and myosin motors is required for intracellular transport, cell motility and mitosis. However, comprehensive resources that allow systems analysis of the localization and interplay between motors in living cells do not exist. Here, we generated a library of 243 amino- and carboxy-terminally tagged mouse and human bacterial artificial chromosome transgenes to establish 227 stably transfected HeLa cell lines, 15 mouse embryonic stem cell lines and 1 transgenic… Expand
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