A genomic approach to isoflavone biosynthesis in kudzu (Pueraria lobata)

  title={A genomic approach to isoflavone biosynthesis in kudzu (Pueraria lobata)},
  author={Xianzhi He and Jack W. Blount and Shujun Ge and Yuhong Tang and Richard A. Dixon},
Roots of kudzu (Pueraria lobata) are a rich source of isoflavone O- and C-glycosides. Although O-glycosylation of (iso)flavonoids has been well characterized at the molecular level, no plant isoflavonoid C-glycosyltransferase genes have yet been isolated. To address the biosynthesis of kudzu isoflavonoids, we generated 6,365 high-quality expressed sequence tags (ESTs) from a subtraction cDNA library constructed using RNA from roots that differentially accumulate puerarin. The ESTs were… CONTINUE READING
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