A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast.

@article{Prszyski2005AGV,
  title={A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast.},
  author={Tomasz Jacek Pr{\'o}szyński and Robin W. Klemm and Maike Gravert and Peggy P. Hsu and Yvonne Gloor and Jan Wagner and Karol Kozak and Hannes Grabner and Karen Walzer and Michel Bagnat and Kai Simons and Christiane Walch-Solimena},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 50},
  pages={17981-6}
}
Recently synthesized proteins are sorted at the trans-Golgi network into specialized routes for exocytosis. Surprisingly little is known about the underlying molecular machinery. Here, we present a visual screen to search for proteins involved in cargo sorting and vesicle formation. We expressed a GFP-tagged plasma membrane protein in the yeast deletion library and identified mutants with altered marker localization. This screen revealed a requirement of several enzymes regulating the synthesis… CONTINUE READING

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