A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture

@inproceedings{OGeen2015AGA,
  title={A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture},
  author={Henriette O'Geen and Isabelle M Henry and Mital S. Bhakta and Joshua F. Meckler and David J Segal},
  booktitle={Nucleic acids research},
  year={2015}
}
  • Henriette O'Geen, Isabelle M Henry, +2 authors David J Segal
  • Published in Nucleic acids research 2015
  • Medicine, Biology
  • Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases have gathered considerable excitement as a tool for genome engineering. However, questions remain about the specificity of target site recognition. Cleavage specificity is typically evaluated by low throughput assays (T7 endonuclease I assay, target amplification followed by high-throughput sequencing), which are limited to a subset of potential off-target sites. Here, we used ChIP-seq to examine genome-wide… CONTINUE READING

    Create an AI-powered research feed to stay up to date with new papers like this posted to ArXiv

    Citations

    Publications citing this paper.
    SHOWING 1-10 OF 26 CITATIONS

    Target specificity of the CRISPR-Cas9 system

    VIEW 6 EXCERPTS
    CITES BACKGROUND & RESULTS
    HIGHLY INFLUENCED

    Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling

    VIEW 1 EXCERPT
    CITES BACKGROUND

    Deep learning improves the ability of sgRNA off-target propensity prediction

    VIEW 1 EXCERPT
    CITES BACKGROUND

    ChIPSeq Analysis with Bayesian Machine Learning

    DNA stretching induces Cas9 off-target activity

    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 53 REFERENCES

    Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

    VIEW 9 EXCERPTS
    HIGHLY INFLUENTIAL

    DNA targeting specificity of RNA-guided Cas9 nucleases

    VIEW 12 EXCERPTS
    HIGHLY INFLUENTIAL

    An encyclopedia of mouse DNA elements (Mouse ENCODE)

    VIEW 5 EXCERPTS
    HIGHLY INFLUENTIAL

    Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

    VIEW 5 EXCERPTS
    HIGHLY INFLUENTIAL

    Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

    VIEW 8 EXCERPTS
    HIGHLY INFLUENTIAL

    High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

    VIEW 4 EXCERPTS
    HIGHLY INFLUENTIAL