A genome-based approach for the identification of essential bacterial genes

@article{Arigoni1998AGA,
  title={A genome-based approach for the identification of essential bacterial genes},
  author={Fabrizio Arigoni and Francois Talabot and Manuel C. Peitsch and Michael D. Edgerton and Eric Meldrum and Elisabeth Allet and Richard Fish and Therese Jamotte and M L Curchod and Hannes Loferer},
  journal={Nature Biotechnology},
  year={1998},
  volume={16},
  pages={851-856}
}
We have used comparative genomics to identify 26 Escherichia coli open reading frames that are both of unknown function (hypothetical open reading frames or y-genes) and conserved in the compact genome of Mycoplasma genitalium. Not surprisingly, these genes are broadly conserved in the bacterial world. We used a markerless knockout strategy to screen for essential E. coli genes. To verify this pheno-type, we constructed conditional mutants in genes for which no null mutants could be obtained… CONTINUE READING