A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance.

@article{Zhang2011AGI,
  title={A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance.},
  author={Meng Zhang and Shixian Lin and Xinwen Song and Jun Liu and Ye Fu and Xi Ge and Xinmiao Fu and Zengyi Chang and Peng R Chen},
  journal={Nature chemical biology},
  year={2011},
  volume={7 10},
  pages={671-7}
}
Acid chaperones are essential factors in preserving the protein homeostasis for enteric pathogens to survive in the extremely acidic mammalian stomach (pH 1-3). The client proteins of these chaperones remain largely unknown, primarily because of the exceeding difficulty of determining protein-protein interactions under low-pH conditions. We developed a genetically encoded, highly efficient protein photocrosslinking probe, which enabled us to profile the in vivo substrates of a major acid… CONTINUE READING