A genetically encoded photocaged amino acid.

  title={A genetically encoded photocaged amino acid.},
  author={Ning Wu and Alexander Deiters and T. Ashton Cropp and David King and Peter G. Schultz},
  journal={Journal of the American Chemical Society},
  volume={126 44},
We have developed a second orthogonal tRNA/synthetase pair for use in yeast based on the Escherichia coli tRNALeu/leucyl tRNA-synthetase pair. Using a novel genetic selection, we have identified a series of synthetase mutants that selectively charge the amber suppresor tRNA with the C8 amino acid, alpha-aminocaprylic acid, and the photocaged amino acid, o-nitrobenzyl cysteine, allowing them to be inserted into proteins in yeast in response to the amber nonsense codon, TAG.