A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578

@inproceedings{Shen2018AGE,
  title={A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578},
  author={Yi Shen and Hod Dana and Ahmed S Abdelfattah and Ronak Patel and Jamien Shea and Rosana S Molina and Bijal Rawal and Vladimir Rancic and Yu-Fen Chang and Lanshi Wu and Yingche Chen and Yong Qian and Matthew D Wiens and Nathan Hambleton and Klaus Ballanyi and Thomas E Hughes and Mikhail Drobizhev and Douglas S Kim and Minoru Koyama and Eric R. Schreiter and Robert E. Campbell},
  booktitle={BMC Biology},
  year={2018}
}
Genetically encoded calcium ion (Ca2+) indicators (GECIs) are indispensable tools for measuring Ca2+ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to green fluorescent protein-based GECIs due to the longer wavelength light used for excitation. Longer wavelength light is associated with decreased phototoxicity and deeper penetration through tissue. Red GECI can also enable multicolor visualization with blue- or… CONTINUE READING