A genetically encoded, fluorescent indicator for cyclic AMP in living cells

  title={A genetically encoded, fluorescent indicator for cyclic AMP in living cells},
  author={Manuela Zaccolo and Francesca De Giorgi and Charles Y. Cho and Luxin Feng and Tom Knapp and Paul A. Negulescu and Susan S. Taylor and Roger Tsien and Tullio Pozzan},
  journal={Nature Cell Biology},
Cyclic AMP controls several signalling cascades within cells, and changes in the amounts of this second messenger have an essential role in many cellular events. Here we describe a new methodology for monitoring the fluctuations of cAMP in living cells. By tagging the cAMP effector protein kinase A with two suitable green fluorescent protein mutants, we have generated a probe in which the fluorescence resonance energy transfer between the two fluorescent moieties is dependent on the levels of… CONTINUE READING


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