A genetic test for yeast two-hybrid bait competency using RanBPM.

  title={A genetic test for yeast two-hybrid bait competency using RanBPM.},
  author={Chandra L. Tucker and Leslie A. Peteya and Adrianne M. C. Pittman and Jing Zhong},
  volume={182 4},
We describe a simple genetic test for assessing the competency of Gal4-based baits prior to a yeast two-hybrid screen, which allows determination of whether a bait protein is expressed appropriately for an interaction to be detected. The novel test, based on interaction with the protein RanBPM, is easier and more predictive than other methods such as Western blotting, allowing identification of approximately 80% of incompetent baits prior to screening. 

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