A genetic system for direct selection of gene-positive clones during recombinational cloning in yeast.

@article{Noskov2002AGS,
  title={A genetic system for direct selection of gene-positive clones during recombinational cloning in yeast.},
  author={V. N. Noskov and Natalay Kouprina and Sun-Hee Leem and Maxim Y. Koriabine and Jeffrey C. Barrett and Vladimir L. Larionov},
  journal={Nucleic acids research},
  year={2002},
  volume={30 2},
  pages={E8}
}
Transformation-associated recombination (TAR) is a cloning technique that allows specific chromosomal regions or genes to be isolated directly from genomic DNA without prior construction of a genomic library. This technique involves homologous recombination during spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences (hooks). Typically, TAR cloning produces positive YAC recombinants at a frequency of approximately 0.5%; the positive clones… CONTINUE READING

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Radial TAR cloning from the TgAC mouse

M. Humble, N. Kouprina, +6 authors R. E. Cannon
2000

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