A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites.

@article{Williams1992AGM,
  title={A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites.},
  author={Brian D. Williams and Bertold Schrank and Chanh Huynh and Ratna Shownkeen and Robert H. Waterston},
  journal={Genetics},
  year={1992},
  volume={131 3},
  pages={609-24}
}
We devised an efficient genetic mapping system in the nematode Caenorhabditis elegans which is based upon the differences in number and location of the transposable element Tc1 between the Bristol and Bergerac strains. Using the nearly completed physical map of the C. elegans genome, we selected 40 widely distributed sites which contain a Tc1 element in the Bergerac strain, but not in the Bristol strain. For each site a polymerase chain reaction assay was designed that can distinguish between… CONTINUE READING
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