A genetic defect in phosphatidylcholine biosynthesis triggers apoptosis in Chinese hamster ovary cells.

@article{Cui1996AGD,
  title={A genetic defect in phosphatidylcholine biosynthesis triggers apoptosis in Chinese hamster ovary cells.},
  author={Zheng Lie Cui and Martin Houweling and Michael H Chen and Michel Record and Hugues Chap and Dennis E. Vance and François Terc{\'e}},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 25},
  pages={14668-71}
}
We have investigated the cell death of a Chinese hamster ovary mutant (MT-58) with a thermo-sensitive CTP:phosphocholine cytidylyltransferase, the rate-limiting enzyme of the CDP-choline pathway for phosphatidylcholine biosynthesis (Esko, J. D., Wermuth, M. M., and Raetz, C. R. H. (1981) J. Biol. Chem. 256, 7388-7393). After MT-58 cells were shifted to the restrictive temperature of 40 degrees C, the cytidylyltransferase was inactivated immediately leading to a decrease in phosphatidylcholine… CONTINUE READING