A generic strategy for CRISPR-Cas9-mediated gene tagging

  title={A generic strategy for CRISPR-Cas9-mediated gene tagging},
  author={Daniel H. Lackner and Alexia Carr{\'e} and Paloma M. Guzzardo and Carina Banning and Ramu Mangena and Tom Henley and Sarah Oberndorfer and Bianca V. Gapp and Sebastian M.B. Nijman and Thijn R Brummelkamp and Tilmann B{\"u}rckst{\"u}mmer},
  booktitle={Nature communications},
Genome engineering has been greatly enhanced by the availability of Cas9 endonuclease that can be targeted to almost any genomic locus using so called guide RNAs (gRNAs). However, the introduction of foreign DNA sequences to tag an endogenous gene is still cumbersome as it requires the synthesis or cloning of homology templates. Here we present a strategy that enables the tagging of endogenous loci using one generic donor plasmid. It contains the tag of interest flanked by two gRNA recognition… CONTINUE READING
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