A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution

@article{Klenk2016AGS,
  title={A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution},
  author={Christoph Klenk and Janosch Ehrenmann and Marco Sch{\"u}tz and Andreas Pl{\"u}ckthun},
  journal={Scientific Reports},
  year={2016},
  volume={6}
}
Structural and biophysical studies as well as drug screening approaches on G protein-coupled receptors (GPCRs) have been largely hampered by the poor biophysical properties and low expression yields of this largest class of integral membrane proteins. Thermostabilisation of GPCRs by introduction of stabilising mutations has been a key factor to overcome these limitations. However, labelled ligands with sufficient affinity, which are required for selective binding to the correctly folded… 
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