A generic protein purification method for protein complex characterization and proteome exploration

@article{Rigaut1999AGP,
  title={A generic protein purification method for protein complex characterization and proteome exploration},
  author={G. Rigaut and A. Shevchenko and B. Rutz and M. Wilm and M. Mann and B. S{\'e}raphin},
  journal={Nature Biotechnology},
  year={1999},
  volume={17},
  pages={1030-1032}
}
  • G. Rigaut, A. Shevchenko, +3 authors B. Séraphin
  • Published 1999
  • Biology, Medicine
  • Nature Biotechnology
  • We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been… CONTINUE READING
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