A generic capture ELISA for recombinant proteins fused to glutathione S-transferase: validation for HPV serology.

@article{Sehr2001AGC,
  title={A generic capture ELISA for recombinant proteins fused to glutathione S-transferase: validation for HPV serology.},
  author={Peter Sehr and Klaus Ulrich Zumbach and Michael Pawlita},
  journal={Journal of immunological methods},
  year={2001},
  volume={253 1-2},
  pages={
          153-62
        }
}
An enzyme-linked immunosorbent assay (ELISA) system has been developed that uses glutathione crosslinked to casein as capture protein to bind recombinant protein antigens fused to N-terminal glutathione S-transferase (GST). The method allows simple and efficient immobilization and one-step purification of overexpressed recombinant antigens from crude lysates on ELISA plates coated with glutathione casein. Several antigens can be tested in parallel under the same conditions without the need to… CONTINUE READING
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