A general purpose RNA-cleaving DNA enzyme.
@article{Santoro1997AGP,
title={A general purpose RNA-cleaving DNA enzyme.},
author={S. Santoro and G. F. Joyce},
journal={Proceedings of the National Academy of Sciences of the United States of America},
year={1997},
volume={94 9},
pages={
4262-6
}
}An in vitro selection procedure was used to develop a DNA enzyme that can be made to cleave almost any targeted RNA substrate under simulated physiological conditions. The enzyme is comprised of a catalytic domain of 15 deoxynucleotides, flanked by two substrate-recognition domains of seven to eight deoxynucleotides each. The RNA substrate is bound through Watson-Crick base pairing and is cleaved at a particular phosphodiester located between an unpaired purine and a paired pyrimidine residue… CONTINUE READING
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