A general purpose RNA-cleaving DNA enzyme.

@article{Santoro1997AGP,
  title={A general purpose RNA-cleaving DNA enzyme.},
  author={S. Santoro and G. F. Joyce},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 9},
  pages={
          4262-6
        }
}
  • S. Santoro, G. F. Joyce
  • Published 1997
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • An in vitro selection procedure was used to develop a DNA enzyme that can be made to cleave almost any targeted RNA substrate under simulated physiological conditions. The enzyme is comprised of a catalytic domain of 15 deoxynucleotides, flanked by two substrate-recognition domains of seven to eight deoxynucleotides each. The RNA substrate is bound through Watson-Crick base pairing and is cleaved at a particular phosphodiester located between an unpaired purine and a paired pyrimidine residue… CONTINUE READING
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