A general protease digestion procedure for optimal protein sequence coverage and post-translational modifications analysis of recombinant glycoproteins: application to the characterization of human lysyl oxidase-like 2 glycosylation.

@article{Rebecchi2011AGP,
  title={A general protease digestion procedure for optimal protein sequence coverage and post-translational modifications analysis of recombinant glycoproteins: application to the characterization of human lysyl oxidase-like 2 glycosylation.},
  author={Kathryn R. Rebecchi and Eden P. Go and Li Xu and Carrie L. Woodin and Minae Mure and Heather Desaire},
  journal={Analytical chemistry},
  year={2011},
  volume={83 22},
  pages={8484-91}
}
Using recombinant DNA technology for expression of protein therapeutics is a maturing field of pharmaceutical research and development. As recombinant proteins are increasingly utilized as biotherapeutics, improved methodologies ensuring the characterization of post-translational modifications (PTMs) are needed. Typically, proteins prepared for PTM analysis are proteolytically digested and analyzed by mass spectrometry. To ensure full coverage of the PTMs on a given protein, one must obtain… CONTINUE READING
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