A general method for the construction of recombinant vaccinia viruses expressing multiple foreign genes.

@article{Coupar1988AGM,
  title={A general method for the construction of recombinant vaccinia viruses expressing multiple foreign genes.},
  author={Barbara E. H. Coupar and Marion E. Andrew and David Bernard Boyle},
  journal={Gene},
  year={1988},
  volume={68 1},
  pages={
          1-10
        }
}
Plasmid vectors with multiple cloning sites adjacent to a vaccinia virus (VV) promoter were constructed and used to insert a protein coding sequence and a dominant selectable marker into a non-essential region of the VV genome. Recombinant viruses, selected on the basis of expression of the herpes simplex virus (HSV) thymidine kinase gene (tk), were shown to express in infected cells the model gene product, murine major histocompatibility complex (MHC) antigen H-2Kd, by cell-surface binding of… CONTINUE READING

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