A general method for site-specific incorporation of unnatural amino acids into proteins.

@article{Noren1989AGM,
  title={A general method for site-specific incorporation of unnatural amino acids into proteins.},
  author={C. J. Noren and S. J. Anthony-Cahill and M. C. Griffith and Peter G. Schultz},
  journal={Science},
  year={1989},
  volume={244 4901},
  pages={
          182-8
        }
}
A new method has been developed that makes it possible to site-specifically incorporate unnatural amino acids into proteins. Synthetic amino acids were incorporated into the enzyme beta-lactamase by the use of a chemically acylated suppressor transfer RNA that inserted the amino acid in response to a stop codon substituted for the codon encoding residue of interest. Peptide mapping localized the inserted amino acid to a single peptide, and enough enzyme could be generated for purification to… Expand
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