A general method for co-crystallization of concanavalin A with carbohydrates.

@article{Moothoo1999AGM,
  title={A general method for co-crystallization of concanavalin A with carbohydrates.},
  author={Davina Noelle Moothoo and James H. Naismith},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={1999},
  volume={55 Pt 1},
  pages={
          353-5
        }
}
A small grid of conditions has been developed for co-crystallization of the plant lectin concanavalin A (conA) and polysaccharides. Crystals have been obtained of complexes of conA with alpha1-2 mannobiose, 1-methyl alpha1-2 mannobiose, fructose, a trisaccharide and a pentasaccharide. The crystals diffract to resolutions of 1. 75-2.7 A using a copper rotating-anode source. The crystals are grown in the presence of polyethylene glycol 6K [10-20%(w/v)] at around pH 6.0. Optimization for each… CONTINUE READING
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