A general framework for optimization of probes for gene expression microarray and its application to the fungus Podospora anserina

@inproceedings{Bidard2010AGF,
  title={A general framework for optimization of probes for gene expression microarray and its application to the fungus Podospora anserina},
  author={Fr{\'e}d{\'e}rique Bidard and Sandrine Imbeaud and Nancie Reymond and Olivier Lespinet and Philippe Silar and Corinne Clav{\'e} and Herv{\'e} Delacroix and V{\'e}ronique Berteaux-Lecellier and Robert Debuchy},
  booktitle={BMC Research Notes},
  year={2010}
}
The development of new microarray technologies makes custom long oligonucleotide arrays affordable for many experimental applications, notably gene expression analyses. Reliable results depend on probe design quality and selection. Probe design strategy should cope with the limited accuracy of de novo gene prediction programs, and annotation up-dating. We present a novel in silico procedure which addresses these issues and includes experimental screening, as an empirical approach is the best… CONTINUE READING

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