A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast

@article{Noskov2003AGC,
  title={A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast},
  author={V. N. Noskov and Natalay Kouprina and Sun-Hee Leem and Ilia Ouspenski and Jeffrey C. Barrett and Vladimir L. Larionov},
  journal={BMC Genomics},
  year={2003},
  volume={4},
  pages={16 - 16}
}
Transformation-associated recombination (TAR) cloning in yeast is a unique method for selective isolation of large chromosomal fragments or entire genes from complex genomes. The technique involves homologous recombination, during yeast spheroplast transformation, between genomic DNA and a TAR vector that has short (~ 60 bp) 5' and 3' gene targeting sequences (hooks). TAR cloning requires that the cloned DNA fragment carry at least one autonomously replicating sequence (ARS) that can function… CONTINUE READING
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Larionov Segments missing from the draft human genome sequence can be isolated by TAR cloning in yeast

  • N Kouprina, SH Leem, +7 authors JC Barrett
  • EMBO Reports
  • 2003

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