A gene of the major facilitator superfamily encodes a transporter for enterobactin (Enb1p) in Saccharomyces cerevisiae

@article{Heymann2000AGO,
  title={A gene of the major facilitator superfamily encodes a transporter for enterobactin (Enb1p) in Saccharomyces cerevisiae},
  author={Petra Heymann and J. F. Ernst and G{\"u}nther Winkelmann},
  journal={Biometals},
  year={2000},
  volume={13},
  pages={65-72}
}
While in fungi iron transport via hydroxamate siderophores has been amply proven, iron transport via enterobactin is largely unknown. Enterobactin is a catecholate-type siderophore produced by several enterobacterial genera grown in severe iron deprivation. By using the KanMX disruption module in vector pUG6 in a fet3Δ background of Saccharomyces cerevisiae we were able to disrupt the gene YOL158c Sce of the major facilitator super family (MFS) which has been previously described as a gene… CONTINUE READING
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Demonstration of enzymatic hydrolysis of enterobactin and its iron

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