A gene-fusion strategy for stoichiometric and co-localized expression of light-gated membrane proteins

@article{Kleinlogel2011AGS,
  title={A gene-fusion strategy for stoichiometric and co-localized expression of light-gated membrane proteins},
  author={Sonja Kleinlogel and Ulrich Terpitz and Barbara Legrum and Deniz G{\"o}kbuget and Edward S. Boyden and Christian Bamann and Phillip G. Wood and Ernst Bamberg},
  journal={Nature Methods},
  year={2011},
  volume={8},
  pages={1083-1088}
}
The precise co-localization and stoichiometric expression of two different light-gated membrane proteins can vastly improve the physiological usefulness of optogenetics for the modulation of cell excitability with light. Here we present a gene-fusion strategy for the stable 1:1 expression of any two microbial rhodopsins in a single polypeptide chain. By joining the excitatory channelrhodopsin-2 with the inhibitory ion pumps halorhodopsin or bacteriorhodopsin, we demonstrate light-regulated… CONTINUE READING
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