A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif

@article{Sinclair1990AGF,
  title={A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif},
  author={Andrew H. Sinclair and Philippe Berta and Mark S. Palmer and J. Ross Hawkins and Beatrice L. Griffiths and Matthijs J. Smith and Jamie W. Foster and A. -M. Frischauf and Robin Lovell-Badge and Peter N. Goodfellow},
  journal={Nature},
  year={1990},
  volume={346},
  pages={240-244}
}
A search of a 35-kilobase region of the human Y chromosome necessary for male sex determination has resulted in the identification of a new gene. This gene is conserved and Y-specific among a wide range of mammals, and encodes a testis-specific transcript. It shares homology with the mating-type protein, Mc, from the fission yeast Schizosaccharomyces pombe and a conserved DNA-binding motif present in the nuclear high-mobility-group proteins HMG1 and HMG2. This gene has been termed SRY (for sex… Expand
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TLDR
The results of this study suggest that in a diverse array of species SRY-related genes may serve as transcription factors that regulate a variety of developmental pathways, including sex determination. Expand
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TLDR
The coding sequence of SRY in primates is investigated and it is found that evolution has been rapid in the regions flanking the conserved domain. Expand
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TLDR
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TLDR
Results are direct evidence that mouse SRY-like proteins are sequence-specific DNA-binding proteins and identify two amino acids critical to this interaction. Expand
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TLDR
Cloned and compared a Y chromosome-specific region immediately 5' to the SRY open reading frame in several primate species and revealed the presence of several highly conserved motifs that may have a function in the control of SRY expression during fetal development. Expand
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A cDNA encoding a DNA-binding protein has been isolated by screening a mouse testicular expression cDNA library with a concatemer of a 12-bp putative protein-binding element present in the promoterExpand
The sex determining gene of medaka: a Y-specific DM domain gene (DMY) is required for male development
TLDR
These findings strongly suggest that the sex-specific DMY is required for normal testicular development and is a prime candidate for the medaka sex-determining gene. Expand
Molecular Mechanisms of Male Sex Determination
TLDR
Progress may benefit from genetic, genomic- and proteomic-based technologies to delineate the downstream pathway of SRY, and new studies of transgenic XX mice expressing chimeric SRY proteins suggest a powerful methodology to investigate structure-function relationships. Expand
Characterization and mapping of the human SOX4 gene
TLDR
The SOX genes comprise a large family related by homology to the HMG-box region of the testis-determining gene SRY and there is no evidence for clustering of other members of the SOX1,-2, and-3 or SOX4 gene families around the SoX4 locus. Expand
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References

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A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes
A gene mapping to the sex-determining region of the mouse Y chromosome is deleted in a line of XY female mice mutant for Tdy, and is expressed at a stage during male gonadal development consistentExpand
The sex-determining region of the human Y chromosome encodes a finger protein
TLDR
The possibility that the Y-encoded finger protein is the testis-determining factor is discussed, and models of sex determination accommodating the finding of a related locus on the X chromosome are proposed. Expand
Mapping the limits of the human pseudoautosomal region and a candidate sequence for the male-determining gene
TLDR
A test of the prediction that the pseudoautosomal gene MIC2 would lie close to the Y-specific region using long-range restriction mapping techniques concludes that MIC2 is less than 200 kilobases away from Y- specific sequences. Expand
Sequences homologous to ZFY, a candidate human sex-determining gene, are autosomal in marsupials
TLDR
Using the ZFY probe, it is found that the Zfy homologous sequences are not on either the X or the Y chromosome in marsupials, but map to the auto-somes, which implies ZFY is not the primary sex-determining gene in mars upials. Expand
Single-copy DNA sequences specific for the human Y chromosome
TLDR
The construction of a partial cosmid library of the human Y chromosome is reported, isolating 30 unrelated DNA probes that are free of highly repetitive sequences, and examining their reaction pattern on male and female genomic blots. Expand
Comparison of human ZFY and ZFX transcripts.
TLDR
The polymerase chain reaction is used to demonstrate the expression of ZFY and ZFX in a wide range of adult and fetal human tissues and to show that ZFX is expressed from the inactive X chromosome present in human-mouse hybrids. Expand
ZFX has a gene structure similar to ZFY, the putative human sex determinant, and escapes X inactivation
TLDR
Transcription analysis of human-rodent hybrid cell lines containing "inactive" human X chromosomes suggests that ZFX escapes X inactivation, which contradicts the "dosage/X-inactivation" model, which postulated that sex is determined by the total amount of functionally interchangeable ZFY and ZFX proteins. Expand
Characterization and evolution of a single-copy sequence from the human Y chromosome.
TLDR
Sequences homologous to this probe underwent a change in copy number and chromosomal distribution during primate evolution, and were hybridized to highly repeated sequences in both male and female DNAs from old world monkeys. Expand
The pseudoautosomal boundary in man is defined by an Alu repeat sequence inserted on the Y chromosome
TLDR
The proximal boundary was found to be formed by the insertion of an Alu sequence on the Y chromosome early in the primate lineage, where similarity between the X and Y chromosomes is reduced and which is no longer subject to recombination. Expand
Genetic evidence that ZFY is not the testis-determining factor
TLDR
It is found that in 4 XX males lacking ZFY, there is exchange of Y-specific sequences next to the pseudoautosomal boundary, redefining the region in which TDF must lie. Expand
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