A gel diffusion assay for quantification of pectin methylesterase activity.

@article{Downie1998AGD,
  title={A gel diffusion assay for quantification of pectin methylesterase activity.},
  author={Bruce Downie and Lynnette M A Dirk and Kristen A. Hadfield and Thea A. Wilkins and Alan B Bennett and Kent J Bradford},
  journal={Analytical biochemistry},
  year={1998},
  volume={264 2},
  pages={149-57}
}
Increased binding of ruthenium red to pectin as the number of methyl esters attached to the pectin decreases was used as the basis for a gel diffusion assay for pectin methylesterase (PME, EC 3.1.1.11) activity. The stained zone diameters resulting from the hydrolysis of 0.1% (w/v) 90% esterified pectin in an agarose gel by diffused, commercial PME were log-linear over 4 orders of magnitude, with a minimum detection limit of 3.6 pkatals. Pectin deesterification as the cause for a stained zone… CONTINUE READING
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