A gateway-compatible yeast one-hybrid system.

  title={A gateway-compatible yeast one-hybrid system.},
  author={Bart Deplancke and Denis Dupuy and Marc Vidal and Albertha J M Walhout},
  journal={Genome research},
  volume={14 10B},
Since the advent of microarrays, vast amounts of gene expression data have been generated. However, these microarray data fail to reveal the transcription regulatory mechanisms that underlie differential gene expression, because the identity of the responsible transcription factors (TFs) often cannot be directly inferred from such data sets. Regulatory TFs activate or repress transcription of their target genes by binding to cis-regulatory elements that are frequently located in a gene's… CONTINUE READING


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