A gas-liquid solid phase peptide and protein sequenator.

  title={A gas-liquid solid phase peptide and protein sequenator.},
  author={Rodney M. Hewick and Michael W. Hunkapiller and Leroy E. Hood and William J. Dreyer},
  journal={The Journal of biological chemistry},
  volume={256 15},
A new miniaturized protein and peptide sequenator has been constructed which uses gas phase reagents at the coupling and cleavage steps of the Edman degradation. The sample is embedded in a matrix of Polybrene dried onto a porous glass fiber disc located in a small cartridge-style reaction cell. The protein or peptide, though not covalently attached to the support, is essentially immobile throughout the degradative cycle, since only relatively apolar, liquid phase solvents pass through the cell… CONTINUE READING


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