A gas-chromatographic method for the determination of the total CO 2 content in biological fluids, by means of the head space sampling technique, based on the methane conversion principle.

@article{Stekelenburg1973AGM,
  title={A gas-chromatographic method for the determination of the total CO 2 content in biological fluids, by means of the head space sampling technique, based on the methane conversion principle.},
  author={G. V. van Stekelenburg and G. Koorevaar},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1973},
  volume={44 3},
  pages={
          367-75
        }
}
Abstract A simple and sensitive gas-chromatographic method is presented for the determination of the total carbon dioxide content in biological fluids, which needs a sample volume of 50 μl. Carbon dioxide, present as bicarbonate in the aqueous phase, is transferred into gaseous carbon dioxide by 0.1 N lactic acid, after which one ml of the head space vapour is analysed by means of gas chromatography. After passing the Chromatographic column, carbon dioxide is reduced catalytically into methane… Expand
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References

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Man-made Carbon Dioxide and the “Greenhouse” Effect