A gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion.

Abstract

The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of… (More)
DOI: 10.1128/AEM.01310-12

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@article{Nakayama2012AGM, title={A gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion.}, author={Yoshitaka Nakayama and Kenjiro Yoshimura and Hidetoshi Iida}, journal={Applied and environmental microbiology}, year={2012}, volume={78 15}, pages={5432-4} }