A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated

@article{Young1987AFM,
  title={A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated},
  author={Ellora G. Young and Maureen R. Hanson},
  journal={Cell},
  year={1987},
  volume={50},
  pages={41-49}
}
Sequencing of an open reading frame associated with cytoplasmic male sterility (CMS) in Petunia has revealed a gene fusion (the Pcf gene) containing the 5'-flanking and amino-terminal transmembrane segment of the ATP synthase proteolipid gene (atp9), parts of the cytochrome oxidase subunit II (coxII) coding region, and the carboxyl terminus and 3'-flanking region of an unidentified reading frame (urfS). The coxII region has several small deletions and tandem repeats that remove all of the… Expand
Chimeric mitochondrial genes expressed in the C male-sterile cytoplasm of maize
TLDR
Aberrant recombinations involving the mitochondrial atp9, atp6 and coxII genes have created unique chimeric sequences in the C male0sterile cytoplasm (cms-C) of maize, and their novel arrangements and the strong correlation between these genes and the C type of male sterility suggest such a role. Expand
Mitochondrial gene organization and expression in petunia male fertile and sterile plants.
TLDR
The presence of the nuclear fertility restorer gene affects the abundance of a transcript class with 5' termini--121 nucleotides before the pcf start codon, and greatly reduces the abundanceOf a pcf gene product with apparent molecular mass of 25 kDa which is present in both vegetative and reproductive tissues of CMS plants. Expand
Suppression of cytoplasmic male sterility by nuclear genes alters expression of a novel mitochondrial gene region.
TLDR
Structural analysis of the atp6 gene regions of pol and nap mitochondrial DNAs showed that rearrangements in the pol mitochondrial genome occurring upstream of at p6 have generated a chimeric 224-codon open reading frame, designated orf224, that is cotranscribed with atp 6. Expand
Organization of ATPA coding and 3′ flanking sequences associated with cytoplasmic male sterility in Phaseolus vulgaris L.
SummaryA region of the mitochondrial genome associated with cytoplasmic male sterility (CMS) in Phaseolus vulgaris was flanked by two different repeated sequences designated x and y. The DNA sequenceExpand
Sequence and expression of a fused mitochondrial gene, associated with Petunia cytoplasmic male sterility, compared with normal mitochondrial genes in fertile and sterile plants
TLDR
By comparing recombinant mitochondrial genomes present in Petunia CMS and fertile somatic hybrids, a mitochondrial DNA region associated with the sterility phenotype was identified and a gene fusion (termed Pcf) was identified which contains coding region homologous to an ATP synthase proteolipid gene (atp9). Expand
Identification of a mitochondrial protein associated with cytoplasmic male sterility in petunia.
TLDR
The petunia fused gene is correlated with cytoplasmic male sterility not only by its co-segregation with the phenotype in somatic hybrids, but also by the modification of its expression at the protein level through the action of a nuclear gene that confers fertility. Expand
The petunia restorer of fertility protein is part of a large mitochondrial complex that interacts with transcripts of the CMS-associated locus.
TLDR
Transgenic petunia plants expressing a functional tagged version of Rf revealed that the restorer protein is part of a soluble mitochondrial inner-membrane-associated, RNase-sensitive high-molecular-weight protein complex associated with mRNA derived from the CMS locus. Expand
Characterization of expression of a mitochondrial gene region associated with the Brassica “Polima” CMS: developmental influences
TLDR
The mitochondrial genome of the Polima (pol) male-sterile cytoplasm of Brassica napus contains a chimeric 224-codon open reading frame (orf224) that is located upstream of, and co-transcribed with, the atp6 gene, indicating that the pol CMS is not caused by the absence of an intact, expressed orfB gene. Expand
A NADH dehydrogenase subunit gene is co-transcribed with the abnormal Petunia mitochondrial gene associated with cytoplasmic male sterility
SummaryDNA sequence analysis 3′ to the Petunia S-pcf coding region has resulted in the identification of an open reading frame similar to mammalian mitochondrial genes for subunit 3 of the NADHExpand
Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene
SummaryThe organization and expression of the mitochondrial (mt) genome of fertile, male-sterile and restored lines of Helianthus annuus and of H. petiolaris were compared to identify alterationsExpand
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