A further study on chromosome minimization by protoplast fusion in Aspergillus oryzae

@article{Hara2011AFS,
  title={A further study on chromosome minimization by protoplast fusion in Aspergillus oryzae},
  author={Seiichi Hara and Feng Jie Jin and Tadashi Takahashi and Yasuji Koyama},
  journal={Molecular Genetics and Genomics},
  year={2011},
  volume={287},
  pages={177-187}
}
Our goal in this work was to develop a method to minimize the chromosomes of Aspergillus oryzae, to arrive at a deeper understanding of essential gene functions that will help create more efficient industrially useful strains. In a previous study, we successfully constructed a highly reduced chromosome 7 using multiple large-scale chromosomal deletions (Jin et al. in Mol Genet Genomics 283:1–12, 2010). Here, we have created a further reduced chromosome A. oryzae mutant harboring a reduced… CONTINUE READING

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