A functional study on polymorphism of the ATP-binding cassette transporter ABCG2: critical role of arginine-482 in methotrexate transport.

@article{Mitomo2003AFS,
  title={A functional study on polymorphism of the ATP-binding cassette transporter ABCG2: critical role of arginine-482 in methotrexate transport.},
  author={Hideyuki Mitomo and Ryo Kato and Akiko Ito and Shiho Kasamatsu and Yoji Ikegami and Isao Kii and Akira Kudo and Eiry Kobatake and Yasuhiro Sumino and Toshihisa Ishikawa},
  journal={The Biochemical journal},
  year={2003},
  volume={373 Pt 3},
  pages={767-74}
}
Overexpression of the ATP-binding cassette transporter ABCG2 reportedly causes multidrug resistance, whereas altered drug-resistance profiles and substrate specificity are implicated for certain variant forms of ABCG2. At least three variant forms of ABCG2 have been hitherto documented on the basis of their amino acid moieties (i.e., arginine, glycine and threonine) at position 482. In the present study we have generated those ABCG2 variants by site-directed mutagenesis and expressed them in… CONTINUE READING