A functional screen for Myc-responsive genes reveals serine hydroxymethyltransferase, a major source of the one-carbon unit for cell metabolism.

@article{Nikiforov2002AFS,
  title={A functional screen for Myc-responsive genes reveals serine hydroxymethyltransferase, a major source of the one-carbon unit for cell metabolism.},
  author={Mikhail A Nikiforov and Sanjay J. Chandriani and Brenda C. O'Connell and Oleksi Petrenko and Iulia E. Kotenko and Andrew Beavis and John M. Sedivy and Michael D. Cole},
  journal={Molecular and cellular biology},
  year={2002},
  volume={22 16},
  pages={5793-800}
}
A cDNA library enriched with Myc-responsive cDNAs but depleted of myc cDNAs was used in a functional screen for growth enhancement in c-myc-null cells. A cDNA clone for mitochondrial serine hydroxymethyltransferase (mSHMT) that was capable of partial complementation of the growth defects of c-myc-null cells was identified. Expression analysis and chromatin immunoprecipitation demonstrated that mSHMT is a direct Myc target gene. Furthermore, a separate gene encoding the cytoplasmic isoform of… CONTINUE READING